Sample Editor (PDX or In Vitro Culture)

Sample

Patient ID

727343

Specimen ID

253-R

Sample ID

J1-PDC

Disease Body Location

Breast

OncoTree Code

BRCA

CTEP SDC Code

10006190 - Invasive breast carcinoma

PDM Type

PDC: Mixed Tumor Culture

Required Media

Complete DMEM/F12

Culture Origin

PDX-Derived Organoid

FACS
Characterization

mMHC-, hHLA+, mCD9-, hCD9+; hEpCAM+, hCD90-, hCD24+ low

Growth Properties

Adherent Monolayer

Proliferation Rate
in Complete Media

24 hrs

Sub-culture
Recommendations

Split ratio: 1:4 to 1:5

Culture Derivation

Prepared from a PDX-derived organoid culture. Determined to be a tumor by FACS analysis, QRT-PCR, cell morphology, and positive tumorigenicity.

Tumorigenic in
NSG Mice

Spheroid Growth

Spheroid Growth
Notes

Soft Agar Growth

Soft Agar
Growth Notes

Fibroblast qRT-PCR
C1 Corr

0.02

Fibroblast qRT-PCR
C2 Corr

-0.04

Fibroblast qRT-PCR
Top Hits

EPCAM,KRT8,KRT18,KRT19,KRT7

Images (if available)

In Vitro Images

View	Image Type	Notes	In Vitro Image
	Light Microscopy	20x magnification, Passage 23
	Xenograft from In Vitro Culture	20X image of cell culture-derived xenograft

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Genomic Analysis

External Genetic Analysis Data

A data file is available if a Download link is displayed:

2.0.2.51.0

2.0.2.51.0

2.0.2.21.0

Download RSEM (genes)

Download RSEM (isoforms)

Ver:

2.0.2.21.0

OncoKB Gene Panel

View	Hugo Symbol	HGVS Protein Change	Variant Allele Frequency	Total Reads	Variant Class	Oncogenicity	Predicted Functional Effect	OncoKB Version
	PIK3CA	p.C420R	0.5520	221	Missense_Mutation	Oncogenic	Gain-of-function	v4.4
	TP53	p.Y236del	1.0000	172	In_Frame_Del	Likely Oncogenic	Likely Loss-of-function	v4.4

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