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Patient ID
349418
Open Patient
Specimen ID
098-R
Open Specimen
Sample ID
PDC
Disease Body Location
Respiratory/Thoracic
OncoTree Code
LUAD
CTEP SDC Code
10025032 - Lung adenocarcinoma
PDM Type
PDC: Mixed Tumor Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM+, hCD90-; hCD24- (note very low amount present)
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
25.5 hrs
Sub-culture
Recommendations
1:3 ratio
Culture Derivation
Isolated from a patient resection. Two separate EpCAM positive sorts were performed. Determined to be a tumor by FACS analysis, low correlation coefficient with the fibroblast Q RT PCR, cell morphology and positive tumorigenicity test.
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.37
Fibroblast qRT-PCR
C2 Corr
0.37
Fibroblast qRT-PCR
Top Hits
KRT7, KRT18, KRT19, KRT8, VIM, EPCAM, CD44, KRT16, KRT13, LOX
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
[20X]: cells grown on plastic as adherent monolayer
Xenograft from In Vitro Culture
20x. H&E in vitro culture-derived PDX
Export
Genomic Analysis
External Genetic Analysis Data
A data file is available if a Download link is displayed:
Somatic Mutations Associated with Cancer (*.vcf and *.maf)
Whole Exome Sequencing (*.vcf)
Download
Ver:
2.0.1.50.0
Whole Exome FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
2.0.2.51.0
RNASeq FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
2.0.2.4.0
RNASeq Transcripts per Million (TPM; *.RSEM.genes.results and *.RSEM.isoforms.results tab-delimited text file)
Download RSEM (genes)
Download RSEM (isoforms)
Ver:
2.0.2.4.0
OncoKB Gene Panel
View
Hugo
Symbol
HGVS Protein
Change
Variant Allele
Frequency
Total
Reads
Variant
Class
Oncogenicity
Predicted
Functional Effect
OncoKB
Version
ARID1A
p.S2249*
1.0000
309
Nonsense_Mutation
Likely Oncogenic
Likely Loss-of-function
v7.1
BRAF
p.V600E
0.4894
47
Missense_Mutation
Oncogenic
Gain-of-function
v7.1
ERF
p.Q263*
0.4800
50
Nonsense_Mutation
Likely Oncogenic
Loss-of-function
v7.1
Export
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