Sample Editor (PDX or In Vitro Culture)

Sample

Patient ID

176247

Specimen ID

063-R

Sample ID

J2-PDC

Disease Body Location

Digestive/Gastrointestinal

OncoTree Code

COAD

CTEP SDC Code

10009951 - Adenocarcinoma - colon

PDM Type

PDC: Mixed Tumor Culture

Required Media

Complete DMEM/F12

Culture Origin

PDX-Derived Organoid

FACS
Characterization

mMHC-, hHLA+, mCD9-, hCD9+; hEpCAM+, hCD90-, hCD24+

Growth Properties

Adherent Monolayer

Proliferation Rate
in Complete Media

65 hrs

Sub-culture
Recommendations

Split ratio: 1:3 to 1:4

Culture Derivation

Prepared from a PDX-derived organoid culture. Determined to be a tumor by FACS analysis, QRT-PCR, cell morphology, and positive tumorigenicity.

Tumorigenic in
NSG Mice

Spheroid Growth

Spheroid Growth
Notes

Soft Agar Growth

Soft Agar
Growth Notes

Fibroblast qRT-PCR
C1 Corr

-0.39

Fibroblast qRT-PCR
C2 Corr

-0.45

Fibroblast qRT-PCR
Top Hits

EPCAM,KRT8,CD24,KRT18,KRT19

Images (if available)

In Vitro Images

View	Image Type	Notes	In Vitro Image
	Light Microscopy	20x magnification, Passage 32
	Xenograft from In Vitro Culture	20X image of cell culture-derived xenograft

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Genomic Analysis

External Genetic Analysis Data

A data file is available if a Download link is displayed:

2.0.2.51.0

2.0.2.51.0

2.0.2.21.0

Download RSEM (genes)

Download RSEM (isoforms)

Ver:

2.0.2.21.0

OncoKB Gene Panel

View	Hugo Symbol	HGVS Protein Change	Variant Allele Frequency	Total Reads	Variant Class	Oncogenicity	Predicted Functional Effect	OncoKB Version
	APC	p.T1368Kfs*4	1.0000	101	Frame_Shift_Del	Likely Oncogenic	Likely Loss-of-function	v7.1
	TP53	p.R175H	1.0000	169	Missense_Mutation	Oncogenic	Loss-of-function	v7.1

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