Sample Editor (PDX or In Vitro Culture)

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NIH, National Cancer

Institute, Division of Cancer Treatment and Diagnosis (DCTD)

Sample

414838

212-R

Value Required

J2-PDC

Disease Body Location

Breast

IDC

10006190 - Invasive breast carcinoma

Value Required

PDC: Mixed Tumor Culture

Breast #2

PDX-Derived Organoid

FACS
Characterization

mMHC-, hHLA+, mCD9-, hCD9+; hEpCAM+, hCD90-, hCD24+

Growth Properties

Adherent Monolayer

Proliferation Rate
in Complete Media

43 hrs

Sub-culture
Recommendations

Split ratio: 1:4 to 1:5

Culture Derivation

Prepared from a PDX-derived organoid culture. Determined to be a tumor by FACS analysis, QRT-PCR, and cell morphology.

Tumorigenic in
NSG Mice

Spheroid Growth

Spheroid Growth
Notes

Soft Agar Growth

Soft Agar
Growth Notes

Not Determined

Fibroblast qRT-PCR
C1 Corr

-0.22

Fibroblast qRT-PCR
C2 Corr

-0.22

Fibroblast qRT-PCR
Top Hits

KRT19,KRT17,CD24,KRT7,KRT16

Hide or show region

Images (if available)

Genomic Analysis

External Genetic Analysis Data

A data file is available if a Download link is displayed:

2.0.4.51.0

Download Read1 FASTQ

Download Read2 FASTQ

2.0.4.51.0

Download Read1 FASTQ

Download Read2 FASTQ

2.0.2.21.0

Download RSEM (genes)

Download RSEM (isoforms)

2.0.2.21.0

OncoKB Gene Panel

View	Hugo Symbol	HGVS Protein Change	Variant Allele Frequency	Total Reads	Variant Class	Oncogenicity	Predicted Functional Effect	OncoKB Version
	CARD11	p.R423W	0.3298	470	Missense_Mutation	Likely Oncogenic	Unknown	v4.16
	KMT2C	p.X338_splice	0.1208	629	Splice_Site	Likely Oncogenic	Likely Loss-of-function	v4.16
	RAD51C	p.A126T	1.0000	205	Missense_Mutation	Likely Neutral	Neutral	v4.16
	TP53	p.G266V	1.0000	69	Missense_Mutation	Likely Oncogenic	Likely Loss-of-function	v4.16
	CHD4	p.E139del	0.0780	372	In_Frame_Del	Inconclusive	Inconclusive	v4.16

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