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Patient ID
589616
Open Patient
Specimen ID
265-R
Open Specimen
Sample ID
J2-PDC
Disease Body Location
Musculoskeletal
OncoTree Code
MPNST
CTEP SDC Code
10026667 - Malig. periph. nerve sheath tum.
PDM Type
PDC: Mixed Tumor Culture
Required Media
Complete DMEM/F12
Culture Origin
PDX Fragment
FACS
Characterization
mMHC-, hHLA+, mCD9-, hCD9+very low; hEpCAM-, hCD90+, hCD24+
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
53 hrs
Sub-culture
Recommendations
Split ratio: 1:3 to 1:4
Culture Derivation
Prepared from passage 5 PDX materials. Mouse cells were depleted from culture using magnetic beads labeled with anti-mCD9 and anti-mMHC antibodies. Determined to be a tumor by FACS analysis, QRT-PCR, cell morphology, and positive tumorigenicity.
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
N/D
Fibroblast qRT-PCR
C1 Corr
0.86
Fibroblast qRT-PCR
C2 Corr
0.85
Fibroblast qRT-PCR
Top Hits
FN1,VIM,COL3A1,KRT18,CD24
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
20x magnification, Passage 22
Xenograft from In Vitro Culture
20X image of cell culture-derived xenograft
Export
Genomic Analysis
External Genetic Analysis Data
A data file is available if a Download link is displayed:
Somatic Mutations Associated with Cancer (*.vcf and *.maf)
Whole Exome Sequencing (*.vcf)
Download
Ver:
2.0.4.51.0
Whole Exome FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
2.0.4.51.0
RNASeq FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
2.0.4.21.0
RNASeq Transcripts per Million (TPM; *.RSEM.genes.results and *.RSEM.isoforms.results tab-delimited text file)
Download RSEM (genes)
Download RSEM (isoforms)
Ver:
2.0.4.21.0
OncoKB Gene Panel
View
Hugo
Symbol
HGVS Protein
Change
Variant Allele
Frequency
Total
Reads
Variant
Class
Oncogenicity
Predicted
Functional Effect
OncoKB
Version
EED
p.X287_splice
1.0000
87
Splice_Site
Likely Oncogenic
Likely Loss-of-function
v7.1
TP53
p.P153Afs*28
1.0000
168
Frame_Shift_Ins
Likely Oncogenic
Likely Loss-of-function
v7.1
CHD4
p.E139del
0.0870
276
In_Frame_Del
Inconclusive
Inconclusive
v7.1
Export
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