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Patient Specimen
Sample (PDX or In Vitro Culture)
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Patient ID
126254
Open Patient
Specimen ID
015-R
Open Specimen
Sample ID
J1-PDC
Disease Body Location
Musculoskeletal
OncoTree Code
MFH
CTEP SDC Code
10039494 - Non-Rhabdo. soft tissue sarcoma
PDM Type
PDC: Mixed Tumor Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
43.4 hrs
Sub-culture
Recommendations
Split 1:5 ratio
Culture Derivation
Isolated from a patient resection. Determined to be a tumor by FACS analysis, qRT-PCR, cell morphology and positive tumorigenicity test.
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.58
Fibroblast qRT-PCR
C2 Corr
0.63
Fibroblast qRT-PCR
Top Hits
FN1, CD248, VIM, KRT7, ERBB2, GREM1, CDH2, KRT5, EGF, KRT8
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
Phase contrast image [20X]: cells grown on plastic as adherent monolayer
Xenograft from In Vitro Culture
Pathology confirmed.
Export
Genomic Analysis
External Genetic Analysis Data
A data file is available if a Download link is displayed:
Somatic Mutations Associated with Cancer (*.vcf and *.maf)
Whole Exome Sequencing (*.vcf)
Download
Ver:
2.0.1.50.0
Whole Exome FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
1.2
RNASeq FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
1.2
RNASeq Transcripts per Million (TPM; *.RSEM.genes.results and *.RSEM.isoforms.results tab-delimited text file)
Download RSEM (genes)
Download RSEM (isoforms)
Ver:
2.0.1.13.0
OncoKB Gene Panel
View
Hugo
Symbol
HGVS Protein
Change
Variant Allele
Frequency
Total
Reads
Variant
Class
Oncogenicity
Predicted
Functional Effect
OncoKB
Version
RB1
p.E428Ifs*3
1.0000
71
Frame_Shift_Del
Likely Oncogenic
Likely Loss-of-function
v3.14
Export
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