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Patient ID
693688
Open Patient
Specimen ID
280-R
Open Specimen
Sample ID
CAF
Disease Body Location
Endocrine and Neuroendocrine
OncoTree Code
SCUP
CTEP SDC Code
10041056 - Small cell car. (extrapulmonary)
PDM Type
CAF Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
60.3 hrs
Sub-culture
Recommendations
Split ratio: 1 : 3. Matrigel-coated plates only
Culture Derivation
Isolated from a patient resection. Sorted three times for CD90 positive cells. Determined to be a fibroblast by qRT-PCR, FACS analysis, cell morphology and lack of tumorigenicity
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.90
Fibroblast qRT-PCR
C2 Corr
0.82
Fibroblast qRT-PCR
Top Hits
FN1, GREM1, CD44, VIM, COL1A1, LOX, COL3A1, COL1A2, ACTA2, CD248
Images (if available)
In Vitro Images
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Image Type
Notes
In Vitro Image
Light Microscopy
Phase contrast image [20X]: cells grown on plastic as adherent monolayer
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