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Patient ID
126779
Open Patient
Specimen ID
323-R
Open Specimen
Sample ID
CAF
Disease Body Location
Genitourinary
OncoTree Code
BLCA
CTEP SDC Code
10018192 - Urothelial/bladder cancer, NOS
PDM Type
CAF Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
34 hrs
Sub-culture
Recommendations
Split ratio: 1 : 3. Matrigel-coated plates only
Culture Derivation
Isolated from a patient biopsy. Determined to be a fibroblast by qRT-PCR, FACS analysis, cell morphology and lack of tumorigenicity
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.93
Fibroblast qRT-PCR
C2 Corr
0.93
Fibroblast qRT-PCR
Top Hits
COL1A1, COL3A1, FN1, COL1A2, GREM1, VIM, ERBB2, LOX, CD248, KRT8
Images (if available)
In Vitro Images
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Image Type
Notes
In Vitro Image
Light Microscopy
Phase contrast image [20X]: cells grown on plastic as adherent monolayer
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