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Patient ID
176247
Open Patient
Specimen ID
063-R
Open Specimen
Sample ID
J1-CAF
Disease Body Location
Digestive/Gastrointestinal
OncoTree Code
COAD
CTEP SDC Code
10009951 - Adenocarcinoma - colon
PDM Type
CAF Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+/- 2 populations; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
27.2 hrs
Sub-culture
Recommendations
Split ratio: 1 : 5. Matrigel-coated plates only
Culture Derivation
Isolated from a patient resection. Determined to be a fibroblast by FACS analysis, QRT-PCR, cell morphology and negative tumorigenicity test.
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.94
Fibroblast qRT-PCR
C2 Corr
0.90
Fibroblast qRT-PCR
Top Hits
FN1, GREM1, COL1A1, COL3A1, VIM, LOX, COL1A2, CD44, ACTA2, KRT8
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
Phase contrast image [20X]: cells grown on plastic as adherent monolayer
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