Skip to Content
Contact PDMR
|
PDMR Database User Guide
Home
Patient
Patient Specimen
Sample (PDX or In Vitro Culture)
Distribution Lots
Genomic Analysis
Reports
Search
Sample
Cancel
Patient ID
115264
Open Patient
Specimen ID
343-R
Open Specimen
Sample ID
CAF
Disease Body Location
Endocrine and Neuroendocrine
OncoTree Code
NETNOS
CTEP SDC Code
10057270 - Neuroendocrine cancer, NOS
PDM Type
CAF Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
68 hrs
Sub-culture
Recommendations
Split ratio: 1 : 2. Matrigel-coated plates only
Culture Derivation
Isolated from a patient resection. Cells were sorted twice for CD90 positive cells. Determined to be a fibroblast by qRT-PCR, FACS analysis, cell morphology and lack of tumorigenicity
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.95
Fibroblast qRT-PCR
C2 Corr
0.88
Fibroblast qRT-PCR
Top Hits
FN1, COL1A1, GREM1, VIM, LOX, COL1A2, COL3A1, KRT18, ACTA2, CD44
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
Cells grown on plastic as adherent monolayer
Export