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Patient ID
119177
Open Patient
Specimen ID
322-R1
Open Specimen
Sample ID
J1-CAF
Disease Body Location
Musculoskeletal
OncoTree Code
SYNS
CTEP SDC Code
10042866 - Synovial sarcoma
PDM Type
CAF Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
50 hrs
Sub-culture
Recommendations
Split ratio: 1 : 3 to 1:4. Matrigel coated flasks only
Culture Derivation
Isolated from a patient resection. Determined to be a fibroblast by FACS analysis, QRT-PCR, cell morphology and a negative tumorigenicity test
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.78
Fibroblast qRT-PCR
C2 Corr
0.74
Fibroblast qRT-PCR
Top Hits
VIM,FN1,COL1A1,COL3A1,KRT8
Images (if available)
In Vitro Images
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Image Type
Notes
In Vitro Image
Light Microscopy
20x magnification, Passage 19
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