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Patient ID
833975
Open Patient
Specimen ID
119-R
Open Specimen
Sample ID
CAF
Disease Body Location
Digestive/Gastrointestinal
OncoTree Code
PAAD
CTEP SDC Code
10052747 - Adenocarcinoma - pancreas
PDM Type
CAF Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
45 hrs
Sub-culture
Recommendations
Split ratio: 1 : 3. Matrigel-coated plates only
Culture Derivation
Isolated from a resection. Determined to be a fibroblast by FACS analysis, QRT-PCR, cell morphology and a negative tumorigenicity test
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.95
Fibroblast qRT-PCR
C2 Corr
0.93
Fibroblast qRT-PCR
Top Hits
FN1 ,COL1A1 ,GREM1 ,COL1A2 , VIM , ACTA2 , COL3A1 ,LOX , CD44 ,KRT18
Images (if available)
In Vitro Images
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Image Type
Notes
In Vitro Image
Light Microscopy
20x magnification, Passage 18
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