Sample Editor (PDX or In Vitro Culture)

Isolated from passage 6 PDX material. Mouse contamination removed by negative sort for murine CD9. Determined to be a tumor by FACS analysis, QRT-PCR, cell morphology and a positive tumorigenicity test.

Tumorigenic in
NSG Mice

Spheroid Growth

Spheroid Growth
Notes

irregularly sized spheroids

Soft Agar Growth

Soft Agar
Growth Notes

Cells form colonies in soft agar; large colonies will be visualized within 1.5-2 weeks.

Fibroblast qRT-PCR
C1 Corr

0.95

Fibroblast qRT-PCR
C2 Corr

0.91

Fibroblast qRT-PCR
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Images (if available)

In Vitro Images

View	Image Type	Notes	In Vitro Image
	Light Microscopy	There are 2 morphologies present - a loosely associated ball type structure and a firmly attached elongated cell
	Spheroid Culture	Growth in StemPro media (no serum) results in growth as irregularly sized spheroids
	Xenograft from In Vitro Culture	20x image of cell culture-derived xenograft; Diagnosis confirmed

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Genomic Analysis

External Genetic Analysis Data

A data file is available if a Download link is displayed:

2.0.4.51.0

2.0.4.51.0

2.0.4.21.0

Download RSEM (genes)

Download RSEM (isoforms)

Ver:

2.0.4.21.0

OncoKB Gene Panel

Hugo Symbol	HGVS Protein Change	Variant Allele Frequency	Total Reads	Variant Class	Oncogenicity	Predicted Functional Effect	OncoKB Version
ARID1A	p.S138*	1.0000	142	Nonsense_Mutation	Likely Oncogenic	Likely Loss-of-function	v4.20
BRCA2	p.R2034C	0.5246	122	Missense_Mutation	Likely Neutral	Likely Neutral	v4.20
RB1	p.R358*	1.0000	54	Nonsense_Mutation	Likely Oncogenic	Likely Loss-of-function	v4.20
TP53	p.R248Q	1.0000	214	Missense_Mutation	Likely Oncogenic	Likely Loss-of-function	v4.20

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