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Patient ID
114868
Open Patient
Specimen ID
125-R
Open Specimen
Sample ID
J1-CAF
Disease Body Location
Head and Neck
OncoTree Code
OPHSC
CTEP SDC Code
10034819 - Pharyngeal squam. cell carcinoma
PDM Type
CAF Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
65 hrs
Sub-culture
Recommendations
Split ratio: 1:3 to 1:4. Matrigel-coated flasks only.
Culture Derivation
Isolated from a patient resection. Determined to be a fibroblast by FACS analysis, QRT-PCR, cell morphology and a negative tumorigenicity test
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.94
Fibroblast qRT-PCR
C2 Corr
0.97
Fibroblast qRT-PCR
Top Hits
FN1,VIM,GREM1,COL1A1,COL1A2
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
20x magnification, Passage 15
Spheroid Culture
20x magnification, Passage 9
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