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Patient ID
114474
Open Patient
Specimen ID
138-T
Open Specimen
Sample ID
J1-CAF
Disease Body Location
Digestive/Gastrointestinal
OncoTree Code
PAAD
CTEP SDC Code
10052747 - Adenocarcinoma - pancreas
PDM Type
CAF Culture
Required Media
Complete DMEM/F12
Culture Origin
Patient Tissue
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM-, hCD90+; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
48 hrs
Sub-culture
Recommendations
Split ratio: 1:4 to 1:5. Matrigel-coated flasks only
Culture Derivation
Isolated from a patient resection. Sorted 3 times for hCD90 positive cells. Determined to be a fibroblast by FACS analysis, QRT-PCR, cell morphology and a negative tumorigenicity test
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.95
Fibroblast qRT-PCR
C2 Corr
0.95
Fibroblast qRT-PCR
Top Hits
COL1A1,FN1,VIM,GREM1,LOX
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
20x magnification, Passage 17
Spheroid Culture
20x magnification, Passage 13
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