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Patient ID
114434
Open Patient
Specimen ID
197-R
Open Specimen
Sample ID
J2-PDC
Disease Body Location
Musculoskeletal
OncoTree Code
SFT
CTEP SDC Code
10039494 - Non-Rhabdo. soft tissue sarcoma
PDM Type
PDC: Mixed Tumor Culture
Required Media
Complete DMEM/F12
Culture Origin
PDX Fragment
FACS
Characterization
mMHC-, hHLA+, mCD9-, hCD9+; hEpCAM-, hCD90+, hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
52 hrs
Sub-culture
Recommendations
Split ratio: 1:3 to 1:4
Culture Derivation
Prepared from passage 2 PDX material. Determined to be a tumor by FACS analysis, QRT-PCR, and cell morphology.
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
0.90
Fibroblast qRT-PCR
C2 Corr
0.92
Fibroblast qRT-PCR
Top Hits
FN1,VIM,COL1A1,COL3A1,GREM1
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
20x magnification, Passage 20
Export
Genomic Analysis
External Genetic Analysis Data
A data file is available if a Download link is displayed:
Somatic Mutations Associated with Cancer (*.vcf and *.maf)
Whole Exome Sequencing (*.vcf)
Download
Ver:
2.0.3.51.0
Whole Exome FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
2.0.3.51.0
RNASeq FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
2.0.3.21.0
RNASeq Transcripts per Million (TPM; *.RSEM.genes.results and *.RSEM.isoforms.results tab-delimited text file)
Download RSEM (genes)
Download RSEM (isoforms)
Ver:
2.0.3.21.0
OncoKB Gene Panel
View
Hugo
Symbol
HGVS Protein
Change
Variant Allele
Frequency
Total
Reads
Variant
Class
Oncogenicity
Predicted
Functional Effect
OncoKB
Version
NOTCH1
p.H614Tfs*17
0.1961
306
Frame_Shift_Del
Likely Oncogenic
Likely Loss-of-function
-
TP53
p.S215I
0.9961
258
Missense_Mutation
Likely Oncogenic
Likely Loss-of-function
-
Export
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