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Sample (PDX or In Vitro Culture)
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Patient ID
K00052
Open Patient
Specimen ID
001-T
Open Specimen
Sample ID
J1-PDC
Disease Body Location
Respiratory/Thoracic
OncoTree Code
LUAD
CTEP SDC Code
10025032 - Lung adenocarcinoma
PDM Type
PDC: Mixed Tumor Culture
Required Media
Complete DMEM/F12
Culture Origin
PDX Fragment
FACS
Characterization
mMHC-, hHLA+; mCD9-, hCD9+; hEpCAM+, hCD90-; hCD24-
Growth Properties
Adherent Monolayer
Proliferation Rate
in Complete Media
44 hrs
Sub-culture
Recommendations
Split ratio: 1:3 to 1:4
Culture Derivation
Isolated from passage 0 PDX material. Determined to be a tumor by FACS analysis, QRT-PCR, cell morphology and a positive tumorigenicity test
Tumorigenic in
NSG Mice
Yes
No
Spheroid Growth
Yes
No
Spheroid Growth
Notes
Soft Agar Growth
Yes
No
Soft Agar
Growth Notes
Fibroblast qRT-PCR
C1 Corr
-0.23
Fibroblast qRT-PCR
C2 Corr
-0.30
Fibroblast qRT-PCR
Top Hits
KRT16,VIM,KRT5,KRT18,KRT19
Images (if available)
In Vitro Images
View
Image Type
Notes
In Vitro Image
Light Microscopy
20x magnification, Passage 19
Spheroid Culture
20x magnification, Passage 7
Xenograft from In Vitro Culture
20X image of cell culture-derived xenograft
Export
Genomic Analysis
External Genetic Analysis Data
A data file is available if a Download link is displayed:
Somatic Mutations Associated with Cancer (*.vcf and *.maf)
Whole Exome Sequencing (*.vcf)
Download
Ver:
2.0.2.51.0
Whole Exome FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
2.0.2.51.0
RNASeq FASTQ (*.FASTQ.gz; for paired-end sequence, download both files)
Download Read1 FASTQ
Download Read2 FASTQ
Ver:
2.0.2.4.0
RNASeq Transcripts per Million (TPM; *.RSEM.genes.results and *.RSEM.isoforms.results tab-delimited text file)
Download RSEM (genes)
Download RSEM (isoforms)
Ver:
2.0.2.4.0
OncoKB Gene Panel
View
Hugo
Symbol
HGVS Protein
Change
Variant Allele
Frequency
Total
Reads
Variant
Class
Oncogenicity
Predicted
Functional Effect
OncoKB
Version
None Found
p.S245Lfs*23
0.6261
222
Frame_Shift_Ins
Likely Oncogenic
Likely Loss-of-function
-
ASXL1
p.G646Wfs*12
0.2719
114
Frame_Shift_Ins
Likely Oncogenic
Likely Loss-of-function
-
KRAS
p.G12D
0.6529
605
Missense_Mutation
Oncogenic
Gain-of-function
-
TP53
p.R158L
0.6053
152
Missense_Mutation
Likely Oncogenic
Likely Loss-of-function
-
TP53
p.K139*
0.3631
168
Nonsense_Mutation
Likely Oncogenic
Likely Loss-of-function
-
Export
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