Sample Editor (PDX or In Vitro Culture)

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NIH, National Cancer

Institute, Division of Cancer Treatment and Diagnosis (DCTD)

Sample

727343

253-R

Value Required

V1-organoid

Disease Body Location

Breast

BRCA

10006190 - Invasive breast carcinoma

Value Required

Organoid Culture

Breast #2

PDX Fragment

FACS
Characterization

mMHC-, hHLA+, mCD9-, hCD9+, hCD90-, hEpCAM+, CD24-

Growth Properties

Organoids

Proliferation Rate
in Complete Media

N/A

Sub-culture
Recommendations

1 Frozen Stock Vial can be used to generate: 12 wells in a 24-well plate

Culture Derivation

Derived from passage 0 PDX material. Sorted twice for hCD9 positive cells

Tumorigenic in
NSG Mice

Spheroid Growth

Spheroid Growth
Notes

N/A

Soft Agar Growth

Soft Agar
Growth Notes

N/A

Fibroblast qRT-PCR
C1 Corr

-0.02

Fibroblast qRT-PCR
C2 Corr

-0.07

Fibroblast qRT-PCR
Top Hits

KRT18,KRT8,KRT19,EPCAM,KRT7

Hide or show region

Images (if available)

In Vitro Images

View	Image Type	Notes	In Vitro Image
	Light Microscopy	Image of total well, 1 mm scale bar, Passage 21, 7 days in culture
	Light Microscopy	Magnified image, 500 micron scale bar, Passage 21, 7 days in culture
	Organoid H&E	10X image of embedded Patient-derived organoids
	Xenograft from In Vitro Culture	20X image of cell culture-derived xenograft

Genomic Analysis

External Genetic Analysis Data

A data file is available if a Download link is displayed:

2.0.2.51.0

Download Read1 FASTQ

Download Read2 FASTQ

2.0.2.51.0

Download Read1 FASTQ

Download Read2 FASTQ

2.0.2.16.0

Download RSEM (genes)

Download RSEM (isoforms)

2.0.2.16.0

OncoKB Gene Panel

View	Hugo Symbol	HGVS Protein Change	Variant Allele Frequency	Total Reads	Variant Class	Oncogenicity	Predicted Functional Effect	OncoKB Version
	PIK3CA	p.C420R	0.4612	232	Missense_Mutation	Oncogenic	Gain-of-function	-
	TP53	p.Y236del	1.0000	152	In_Frame_Del	Likely Oncogenic	Likely Loss-of-function	-
	CHD4	p.E139del	0.0831	313	In_Frame_Del	Inconclusive	Inconclusive	-

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